The word 'virgin' shows up in many places, where it symbolizes the Lord's kingdom and the church and therefore anyone who is a kingdom of the Lord, or who is a church. (Arcana Coelestia 3081)

In Genesis 24:16, a virgin signifies purity from all falsity.

In Leviticus 21:13 and Ezekiel 44:22, virgins signify the celestial church and people of the church who love the neighbor from their love of the Lord. That this might be represented in the Jewish Church, the priests were enjoined to marry virgins or the widow of another priest. (Arcana Coelestia 2362[4])

'Virgins,' as in Revelation 14:4, signify people who love truths because they are truths, so from a spiritual affection.

'Virgin' signifies the church as a spouse, who desires to be conjoined to the Lord, and to become a wife. The church that desires this union loves truths because they are truths. When life lived according to truths conjoins. This is why Israel, Zion, and Jerusalem, in the Word, are called 'virgins' and 'daughters' because they represent the church.

2362. That 'behold now, I have two daughters, who have not known a man' means the affections for good and for truth is clear from the meaning of 'daughters' as affections, dealt with in 489-491. 'They have not known a man' means that they have not been defiled by falsity, for 'a man' means rational truth, and also in the contrary sense falsity, 265, 749, 1007. There are two types of affection, namely the affection for good and the affection for truth, see 1997. The first - the affection for good - constitutes the celestial church and in the Word is called 'the daughter of Zion' and also 'the virgin daughter of Zion'.

[2] But the second - the affection for truth - constitutes the spiritual church, and in the Word is called 'the daughter of Jerusalem'; as in Isaiah,

She has despised you, she has scorned you, the virgin daughter of Zion; she wags her head behind you, the daughter of Jerusalem. Isaiah 37:22; 2 Kings 19:21.

In Jeremiah,

What shall I liken you to, O daughter of Jerusalem? What shall I equate you with and comfort you, O virgin daughter of Zion? Lamentations 2:13.

In Micah,

You, O tower of the flock, hill of the daughter of Zion, to you will it come and the former dominion will come, the kingdom of the daughter of Jerusalem. Micah 4:8.

In Zephaniah,

Shout with joy, O daughter of Zion! Make a noise, O Israel! Rejoice and exult with all your heart, O daughter of Jerusalem! Zephaniah 3:14.

In Zechariah,

Exult greatly, O daughter of Zion! Make a noise, O daughter of Jerusalem! Behold, your king will come to you. Zechariah 9:9; Matthew 21:5; John 12:15.

[3] That the celestial Church, which is the Lord's celestial kingdom, is called 'the daughter of Zion' from the affection for good, that is, from love to the Lord Himself, see in addition Isaiah 10:32; 16:1; 52:2; 62:11; Jeremiah 4:31; 6:2, 23; Lamentations 1:6; 2:1, 4, 8, 10; Micah 4:10, 13; Zechariah 2:10; Psalms 9:14. And that the spiritual Church, which is the Lord's spiritual kingdom, is called 'the daughter of Jerusalem' from the affection for truth and so from charity towards the neighbour, see Lamentations 2:15. Both of those Churches, and the nature of each one, have been dealt with many times in Volume One.

[4] Because the celestial Church exists from love to the Lord which is present within love towards the neighbour it is likened in particular to an unmarried daughter or a virgin. Indeed it is also called 'a virgin', as in John,

These are the ones who were not defiled with women, for they are virgins; these are the ones who follow the Lamb wherever He goes; for they are spotless before God's throne. Revelation 14:4-5.

And so that the same might be represented in the Jewish Church, the priests were commanded not to marry widows but virgins, Leviticus 21:13-15; Ezekiel 44:22.

[5] From the contents of the present verse it becomes clear how pure the Word is in the internal sense, however else it may appear in the letter. For when these words are read, 'Behold now, I have two daughters, who have not known a man; let me now bring them out to you and you may do to them as is good in your eyes; only do nothing to those men', nothing else comes to mind than something impure, especially to those leading an evil life. Yet how chaste these words are in the internal sense is evident from the explanation already given, which is that they mean the affections for good and truth and the blessedness perceived from the enjoyment of those affections by people who do no violence to the Lord's Divinity and [proceeding] Holiness.